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目的探究环境类雌激素邻苯二甲酸二丁酯(DBP)诱导建立小鼠隐睾模型的方法及其作用机制。方法将7～8周龄的BALB/c怀孕小鼠40只随机分为对照组(n=10),DBP低剂量诱导组(n=10),DBP中剂量诱导组(n=10),DBP高剂量诱导组(n=10),在怀孕的第12天开始实验组填喂DBP,剂量分别为100mg·kg-1·d-1、300mg·kg-1·d-1、500mg·kg-1·d-1,对照组填喂100mg·kg-1·d-1的玉米油,计算新生小鼠睾丸到膀胱颈的距离与肾下极到膀胱颈距离的比值,并取睾丸组织检测NO合酶(NOS)活性和Caspase-3蛋白水平变化。结果经DBP诱导后,小鼠睾丸到膀胱颈的距离与睾丸到肾下极距离的比值随着DBP浓度的增加显著增大(P<0.05);对照组、低剂量组、中剂量组和高剂量组NOS活性分别为(0.37±0.12)nmol·min-1·g-1、(0.59±0.09)nmol·min-1·g-1、(0.98±0.11)nmol·min-1·g-1、(1.35±0.07)nmol·min-1·g-1,且随着剂量的增加NOS活性显著增加(P<0.05);低剂量组、中剂量组和高剂量组Caspase-3蛋白活化程度分别为1.31±0.36、01.89±0.68、2.71±0.39,且随着剂量的增加Caspase-3蛋白活化程度显著加强(P<0.05)。结论环境类雌激素DBP能够明显诱导小鼠隐睾模型的建立,并显著改变睾丸组织的NOS活性和Caspase-3活化程度。
Objective To investigate the method and mechanism of environmental estrogen dibutyl phthalate (DBP) induced mouse cryptorchid model. Methods Forty BALB / c pregnant mice aged 7-8 weeks were randomly divided into control group (n = 10), DBP low dose group (n = 10), DBP medium dose group (n = 10), DBP In the high-dose induction group (n = 10), DBP was fed to the experimental group on the 12th day of pregnancy with doses of 100 mg · kg-1 · d-1, 300 mg · kg-1 · d-1 and 500 mg · kg- 1 · d-1. The control group was fed with 100 mg · kg-1 · d-1 corn oil to calculate the ratio of the distance from the testicle to the bladder neck and the distance from the lower kidney to the bladder neck, and the testicular tissue was used to detect NO Synthase (NOS) activity and Caspase-3 protein levels. Results After DBP induction, the ratio of testis to bladder neck distance to testicular to renal electrode was significantly increased with the increase of DBP concentration (P <0.05). In control group, low dose group, medium dose group and high The activity of NOS in the dose group were (0.37 ± 0.12) nmol · min-1 · g-1, (0.59 ± 0.09) nmol · min-1 · g-1 and (0.98 ± 0.11) nmol · min-1 · g-1 (1.35 ± 0.07) nmol · min-1 · g-1, and the activity of NOS increased with the increase of dose (P <0.05). The activation of Caspase-3 protein in low dose group, middle dose group and high dose group were 1.31 ± 0.36,01.89 ± 0.68,2.71 ± 0.39, and the activation of Caspase-3 protein increased significantly with the increase of dose (P <0.05). Conclusion Environmental estrogen DBP can significantly induce the establishment of cryptorchidism in mice and significantly change the activity of NOS and the activation of Caspase-3 in testis.