本文就荧光衍生化试剂的新进展作一简介。时间延迟荧光测定法是将非特异性本底荧光消失后测定延迟荧光，提高了方法的选择性和灵敏度。近红外吸收色素的诞生，聚甲炔链中央部导入环化结构，实现了色素的稳定化。利用化学发光衍生化试剂，提高了测定方法的灵敏度。 This article gives a brief introduction of the new progress of fluorescence derivatization reagents. Time delay fluorimetry is the determination of delayed fluorescence after the disappearance of nonspecific background fluorescence, improving the selectivity and sensitivity of the method. The birth of the near-infrared absorbing dye led to the cyclization structure at the central part of the polymethine chain to stabilize the dye. Chemiluminescent derivatization reagents are used to increase the sensitivity of the assay.