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目的观察基质金属蛋白酶MMP-2在新生兔机械通气肺损伤中的表达和变化规律,研究肺损伤的分子机制。方法将108只新生兔随机分配在高浓度氧、低浓度氧组,每组又分为高吸气峰压、中吸气峰压、低吸气峰压共6组进行机械通气并与正常未通气组6只进行对照,通气后1、3、6h3个时间点活杀取肺,病理切片MMP-2免疫组织化学染色,ELISA法检测肺组织匀浆中MMP-2的浓度,RT-PCR检测肺组织MMP-2 mRNA的表达。结果机械通气时,不同浓度的氧能使MMP-2表达上调,高浓度氧组为(5.38±1.57)μg/ml、低浓度氧组为(4.83±1.46)μg/ml,两组间差异无统计学意义,但同对照组(4.36±0.51)μg/ml相比,高氧组差异有统计学意义(F=3.658,P<0.05)。不同的机械拉伸力能使MMP-2上调,高、中、低压力3组分别为(5.10±1.79)、(5.35±1.38)、(4.96±1.49)μg/ml,3组间差异无统计学意义,但同对照组比,中压力组差异有统计学意义(F=2.764,P<0.05)。MMP-2同肺组织湿干比弱相关,同灌洗液细胞总数、细胞分类、肺损伤评分间统计学不相关。结论新生兔机械通气6h内,高浓度的氧通气能上调MMP-2,适中机械拉伸上调MMP-2,MMP-2同肺损伤有较少的相关性。
Objective To investigate the expression of matrix metalloproteinase-2 (MMP-2) in lung tissue of rabbits with mechanical ventilation and its change and to study the molecular mechanism of lung injury. Methods 108 newborn rabbits were randomly assigned to high concentration of oxygen and low concentration of oxygen, each group was divided into high peak inspiratory pressure, peak inspiratory pressure, low peak inspiratory pressure in a total of six groups for mechanical ventilation and normal Ventilation group 6 were controlled, 1, 3, 6h after ventilation, the lungs were sacrificed at 3 time points, and MMP-2 immunohistochemical staining was performed in pathological sections. The concentration of MMP-2 in lung homogenate was detected by ELISA. Expression of MMP-2 mRNA in lung tissue. Results The results showed that different concentrations of oxygen could up-regulate the expression of MMP-2 in high concentration oxygen group (5.38 ± 1.57 μg / ml) and low concentration oxygen group (4.83 ± 1.46 μg / ml) in mechanical ventilation. There was no significant difference between the two groups However, compared with the control group (4.36 ± 0.51) μg / ml, there was significant difference between the two groups (F = 3.658, P <0.05). Different mechanical tensile forces could up-regulate the expression of MMP-2. The high, moderate and low pressures were (5.10 ± 1.79), (5.35 ± 1.38) and (4.96 ± 1.49) μg / However, compared with the control group, there was a significant difference in the medium pressure group (F = 2.764, P <0.05). The wet-dry ratio of MMP-2 was weakly correlated with lung tissue, and it was not correlated with the total number of cells in the lavage fluid, cell classification and lung injury scores. Conclusions Within 6 hours of mechanical ventilation in neonatal rabbits, high concentration of oxygen can up-regulate MMP-2 within 6 hours of mechanical ventilation. Moderate mechanical stretching can up-regulate MMP-2 and MMP-2 with less correlation with lung injury.