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目的探讨以微生物学和分子生物学角度寻找金黄色葡萄球菌引起的食源性疾病的病因。方法分别以食品卫生微生物检验国家标准(GB／T4789)分离鉴定菌株、K-B法进行药敏试验、mini-VIDAS全自动荧光酶标免疫测试仪进行葡萄球菌肠毒素检测和RiboPrinter全自动微生物基因指纹鉴定系统进行基因分型对一起由金黄色匍萄球菌引起的中毒事件进行分析。结果从来源于各类共17份样品的菌株中,均检出葡萄球菌肠毒素,对青霉素G100％耐药,对其他10种抗生素耐药率低于30％,RiboPrinter全自动微生物基因指纹鉴定系统结果证实为一起同型金黄色葡萄球菌的爆发。结论结合微生物学和分子生物学检测结果,确认这起食源性疾病是由产生肠毒素的金黄色葡萄球菌污染食物所引起。
Objective To explore the etiology of food-borne diseases caused by Staphylococcus aureus in terms of microbiology and molecular biology. Methods The strains were isolated and identified by the national standard of food hygiene microbe test (GB / T4789), KB method was used for drug susceptibility test, mini-VIDAS automatic fluorescence enzyme immunoassay was used to detect staphylococcal enterotoxin and RiboPrinter automatic microbial gene fingerprint System Genotyping A poisoning event caused by Staphylococcus aureus was analyzed. Results Staphylococcal enterotoxins were detected in all 17 strains of isolates, resistant to penicillin G 100%, and resistant to 10 other antibiotics. The RiboPrinter automated microbial gene fingerprinting system The results confirmed an outbreak of homotype Staphylococcus aureus. Conclusions Combined with the results of microbiology and molecular biology tests, it was confirmed that this food-borne illness is caused by contaminating food with enterotoxin-producing Staphylococcus aureus.