本文探索了一种在非水介质中对酶进行固定化的新方法.研究了包埋于“水/AOT/异辛烷”系统中与有机溶剂共存的液晶相(L+LC)中的脂肪酶催化橄榄油水解的特性,发现于最适温度28℃,最佳pH为7.2,组成为(w/w%):AOT14.0%、水55.9%、异辛烷15.1%、橄榄油15.0%的条件下,脂肪酶活力较高,并且具有相当好的稳定性,尤其是产物分离和酶的回收简单易行,具有潜在的工业应用前景. This article explores a new method for immobilizing enzymes in nonaqueous media. The effects of lipids embedded in the liquid phase (L + LC) coexisting with organic solvents embedded in a “water / AOT / isooctane” The enzyme catalyzed the hydrolysis of olive oil and was found at an optimum temperature of 28 ° C and an optimum pH of 7.2 with a composition of (w / w%) AOT 14.0%, water 55.9%, isooctane 15.1%, olive oil 15.0% , The lipase activity is high and has good stability. Especially, the separation of the product and the recovery of the enzyme are simple and easy, which has the potential prospect of industrial application.