论文部分内容阅读
目的:以斑马鱼为模型,评价并比较酒石酸长春瑞滨注射用乳剂与市售长春瑞滨水针剂(诺维本)的骨髓毒性、肠道毒性的变化情况。方法:以不同浓度酒石酸长春瑞滨注射用乳剂与水针剂静脉注射给药,观察对斑马鱼红细胞和中性粒细胞数量的影响以及对肠蠕动的影响,比较2种剂型的毒性情况。结果:长春瑞滨乳剂和水针剂均抑制红细胞和中性粒细胞的形成,并呈现明显的剂量效应;相同剂量的乳剂对中性粒细胞形成的抑制作用显著低于水针剂(P<0.001,P<0.01);2种制剂对红细胞形成的抑制作用无统计学差异;与5%葡萄糖注射液组相比,长春瑞滨水针剂显著抑制肠蠕动(P<0.01),并呈明显的剂量效应;相同剂量乳剂组对肠蠕动仅产生轻微的抑制作用(抑制率:4%~7%),与5%葡萄糖注射液组相比无明显差异。结论:与酒石酸长春瑞滨注射用水针剂相比,乳剂的骨髓抑制毒性和肠道毒性较低,提示乳剂具有降低长春瑞滨临床不良反应的优势。
OBJECTIVE: To evaluate the changes of myelotoxicity and intestinal toxicity of vinorelbine tartrate injection and marketed Vinorelbine (Novobibain) by using zebrafish as a model. Methods: The different concentrations of vinorelbine tartrate injection and aqueous injection of intravenous injection, observe the number of red blood cells and neutrophils in zebrafish and the impact on intestinal peristalsis, compared the toxicity of the two formulations. RESULTS: Both vinorelbine and water injection inhibited the formation of erythrocytes and neutrophils, and showed obvious dose effect. The inhibition of neutrophil formation by the same dose of emulsion was significantly lower than that of water injection (P <0.001, P <0.01). Compared with 5% glucose injection group, Vinorelbine injection significantly inhibited bowel movement (P <0.01), and showed a dose-dependent effect ; The same dose of emulsion group only slightly inhibited peristalsis (inhibition rate: 4% to 7%), compared with 5% glucose injection group no significant difference. CONCLUSION: Compared with vinorelbine tartrate injection solution, the mycotic inhibitory and intestinal toxicity of the emulsion is low, suggesting that the emulsion has the advantage of reducing the clinical adverse reactions of vinblastine.