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目的探讨Notch-1和Notch-2在卵巢肿瘤的形成和转化中的表达特点及其潜在肿瘤生物学意义。方法2000-12-2003-06采用人类卵巢肿瘤组织芯片和免疫组织化学染色方法,在完全相同的实验条件下检测各类卵巢肿瘤Notch-1和Notch-2的表达和活化情况。结果Notch-1在50%(3/6)的浆液性囊腺瘤和33·3%(2/6)的粘液性囊腺瘤中有核易位现象;其中,16·7%(1/6)和33·3%(2/6)属于明显易位(++/+++)。在交界性肿瘤中的核易位无论是频率(20%;1/5)还是易位的阳性程度均明显减低,而这种趋势在各类卵巢恶性肿瘤中亦可见到。Mann-WhitnoyU检验显示:Notch-1的核易位在良性和恶性肿瘤之间差异有统计学意义(P<0·05)。Notch-2普遍存在于各类卵巢上皮肿瘤中;其表达水平和核易位情况与肿瘤的良恶性之间无关。结论Notch-1的表达异常和核易位出现在卵巢上皮的恶性转化的过程,它可能是卵巢肿瘤恶变风险和不良预后的生物学标志物。
Objective To investigate the expression characteristics of Notch-1 and Notch-2 in ovarian neoplasms and their potential tumor biomarkers. Methods The expression and activation of Notch-1 and Notch-2 in various ovarian tumors were examined under the same experimental conditions using human ovarian tumor tissue microarray and immunohistochemical staining method from December 2000 to June 2003. Results Notch-1 had nuclear translocation in 50% (3/6) serous cystadenomas and 33.3% (2/6) mucinous cystadenomas. Among them, 16.7% (1 / 6) and 33.3% (2/6) belonged to obvious translocation (++ / +++). Nuclear translocation in borderline tumors was significantly reduced both in frequency (20%; 1/5) and in translocations, a trend that is also seen in various ovarian malignancies. Mann-WhitnoyU test showed that there was a significant difference in nuclear translocation of Notch-1 between benign and malignant tumors (P <0.05). Notch-2 is widespread in various epithelial ovarian tumors; its expression level and nuclear translocation are not related to the benign and malignant tumors. Conclusions The abnormal expression of Notch-1 and nuclear translocation occur in the process of malignant transformation of ovarian epithelial epithelium, which may be the biological marker of ovarian cancer risk of malignancy and poor prognosis.