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为了探索小麦-黑麦双二倍体形成过程中微卫星序列的变异情况,为异源多倍体进化及小麦育种研究提供理论参考,以来源于3个组合的小麦-黑麦杂交后代植株及亲本小麦、黑麦为材料,利用已有的小麦和黑麦微卫星标记调查小麦-黑麦双二倍体形成过程中微卫星序列的变化。结果表明,所用的126对小麦SSR引物中,除22对没有扩增出产物外,其余104对引物从每个组合的后代植株中扩增的带型与从其亲本小麦中扩增的带型完全相同。所用的27对黑麦SSR引物中,10对引物从每个组合的后代植株中扩增的带型与其从亲本黑麦中扩增的带型完全相同,10对引物在亲本黑麦、F1植株及3个双二倍体植株之间表现出了多态性,7对引物从亲本黑麦中扩增的条带在所有后代中缺失。说明小麦-黑麦异源多倍体化过程中小麦微卫星序列是相对稳定的,而黑麦微卫星序列比小麦微卫星序列更易受到异源多倍体化的影响。微卫星序列变化是伴随多倍体体化而快速发生的。
In order to explore the variation of microsatellite sequences during the diploid diploid formation of wheat-rye, it provides a theoretical reference for the research of allopolyploid evolution and wheat breeding. The three wheat-rye hybrid progenies derived from three combinations The parents of wheat and rye were used as materials to investigate the changes of microsatellite sequences during the formation of diploid diploid wheat-rye using the existing wheat and rye microsatellite markers. The results showed that among the 126 pairs of wheat SSR primers used, except that 22 pairs did not amplify the product, the remaining 104 pairs of primers amplified from each combination of progeny plants and the bands amplified from their parents Exactly the same. Of the 27 pairs of rye SSR primers used, 10 pairs of primers were amplified from each combination of progeny plants in exactly the same manner as the bands amplified from the parental rye, with 10 pairs of primers in the parental rye, F1 plants And three diploid plants showed polymorphism, and seven pairs of primers amplified from parental rye were missing in all offspring. The results showed that the wheat microsatellite sequences were relatively stable during the allopolyploidization of wheat-rye while the rye microsatellite sequences were more susceptible to heterologous polyploidization than the wheat microsatellite sequences. Microsatellite sequence changes occur rapidly with polyploidization.