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为研究Graves病患者外周血T细胞亚群及共刺激分子的表达 ,并探讨其在Graves免疫病理机制中的作用 ,本文采用免疫荧光标记和流式细胞仪术检测了 19例Graves病患者和 11例正常人外周血T细胞亚群及共刺激分子 (CD2 8、B7、CD40、CD40L、 4 1BB、OX40 )的表达。结果表明 ,19例初诊Graves病患者外周血T细胞亚群呈现异常改变 ,表现为CD3+ 和CD4+T细胞显著下降 (P <0 0 5 ,P <0 0 1) ,CD4+ /CD8+ T细胞比值倒置 (P <0 0 1) ,共刺激分子CD2 8在T细胞中的表达水平明显下降 (P <0 0 1) ,T细胞亚群CD4+ CD2 8+ 、CD8+ CD2 8+ 细胞数量均减少 (P <0 0 5 ,P <0 0 1) ,而 4 1BB分子表达显著地升高 (P <0 0 5 )。随防 10例治疗后缓解患者 ,T细胞亚群失衡明显改善 ,共刺激分子CD2 8的表达水平上调 (P <0 0 1) ,而4 1BB分子的表达明显下降 (P <0 0 1) ,T细胞亚群CD4+ CD2 8+ 、CD8+ CD2 8+ 细胞数量均增加 (P <0 0 1) ,甲状腺自身抗体甲状腺球蛋白抗体 (TGAb )和甲状腺微粒体抗体 (TMAb )的表达水平均下降 (P <0 0 1,P <0 0 1)。从而表明 ,T细胞亚群的异常及共刺激分子CD2 8、 4 1BB的表达改变与Graves病的免疫病理机制相关。
In order to study the expression of T lymphocyte subsets and costimulatory molecules in peripheral blood of patients with Graves disease and to explore its role in the immunopathological mechanism of Graves, immunofluorescence labeling and flow cytometry were used to detect the expression of 19 T cell subsets and 11 Cases of normal human peripheral blood T cell subsets and costimulatory molecules (CD28, B7, CD40, CD40L, 4 1BB, OX40) expression. The results showed that there were abnormal changes of T lymphocyte subsets in peripheral blood of 19 patients with newly diagnosed Graves’ disease, showing a significant decrease of CD3 + and CD4 + T cells (P <0.05, P <0.01), the ratio of CD4 + / CD8 + T cells was upside down (P <0.01). The expression of costimulatory molecule CD28 decreased significantly in T cells (P <0.01), while the number of CD4 + CD28 + and CD8 + CD28 + cells in T lymphocytes decreased (P < 0 0 5, P <0 01), while 4 1BB expression was significantly increased (P 0 05). The imbalance of T cell subpopulation was significantly improved with the alleviation of 10 patients after treatment. The expression of costimulatory molecule CD28 was up-regulated (P <0.01), while the expression of 4 1BB was significantly decreased (P <0.01) The number of CD4 + CD8 + and CD8 + CD8 + cells in T lymphocyte subsets increased (P <0.01), thyroid autoantibodies TGAb and thyroid microsomal antibody (TMAb) <0 0 1, P <0 0 1). Thus, T cell subsets abnormalities and costimulatory molecules CD2 8, 4 1BB expression changes associated with Graves disease immunopathological mechanisms.