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目的:探讨建立理想糖尿病动物模型的方法。方法:A组采用空腹腹腔一次性注射65mg/kg1%链脲佐菌素(STZ)溶液的方法;B组采用空腹腹腔连续五天注射45mg/kg1%STZ溶液的方法,建立KM小鼠糖尿病模型。结果:建模1周后测血糖,A组平均血糖浓度为16.2mmol/L,死亡率为15%,成模率为55%;B组平均血糖浓度为20.2mmol/L,死亡率为20%,成模率为80%。持续观察一个月,A组血糖值始终在建模初期血糖水平上微弱波动,平均血糖浓度为14.4mmol/L,B组平均血糖浓度一个月后上升至24.8mmol/L,皆未见到复转。结论:采用空腹腹腔一次性注射65mg/kgSTZ溶液的方法复制出糖尿病模型,造模方法简便、用药量小,但成模率较低,血糖水平稳定性较弱;采用空腹腹腔连续五天注射45mg/kgSTZ溶液的方法复制出糖尿病模型,血糖浓度维持在较高水平,成模率高,死亡率较低,可应用于糖尿病及其并发症研究的各个领域。
Objective: To explore the method of establishing an ideal diabetic animal model. Methods: Group A was treated with fasting intraperitoneal injection of 65mg / kg streptozotocin (STZ) solution. In group B, KM mice were established by injecting 45mg / kg 1% STZ solution in fasting abdominal cavity for five consecutive days. . Results: After 1 week of modeling, the average blood glucose concentration was 16.2mmol / L in group A, the mortality rate was 15% and the rate of forming module was 55%. The mean blood glucose concentration in group B was 20.2mmol / L and the mortality rate was 20% , Molding rate of 80%. After a month of continuous observation, the blood glucose level in group A always fluctuated slightly at the initial blood glucose level, the mean blood glucose level was 14.4 mmol / L, and the average blood glucose level in group B increased to 24.8 mmol / L after one month. . CONCLUSION: The model of diabetes mellitus is replicated by the fasting intraperitoneal injection of 65mg / kgSTZ solution. The method of modeling is simple, the dosage is small, but the modulus of formation is low and the stability of blood glucose level is weak. Adopting fasting intraperitoneal injection of 45mg / kgSTZ solution to copy the model of diabetes, blood glucose levels remain at high levels, high molding rate, lower mortality, can be used in various fields of diabetes and its complications.