Polymerization-induced chiral self-assembly (PICSA) is an efficient strategy that not only allows the construction of the supramolecular chiral assemblies in a controlled manner but also can regulate the morphology in situ.Herein,a series of azobenzene-containing block copolymer(Azo-BCP) assemblies with tunable morphologies and supramolecular chirality were obtained through the PICSA strategy.The supramolecular chirality of Azo-BCP assemblies could be regulated by carbon dioxide (CO2) stimulus,and completely recovered by bubbling with Ar.A reversible morphology transformation and chiroptical switching process could also be achieved by the alternative 365 nm UV light irradiation and heating-cooling treatment.Moreover,the supramolecular chirality is thermo-responsive and a reversible chiral-achiral switching was successfully realized,which can be reversibly repeated for at least five times.This work provides a feasible strategy for constructing triple stimuli-responsive supramolecular chiral nano-objects in situ.