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从多种植株根际土壤中分离到 30 0个细菌分离物 ,通过含菌平板培养筛选出X2 ,B3,1J’ ,2e’等近 4 0株 ,对青枯病菌 (马铃薯和烟草 )具有较强的拮抗力 ,室内测定对病原菌抑菌圈达 0 8～ 3 0cm .挑选出 1j’ ,2e’ ,X2和B3进一步进行室内抗菌机理、菌株鉴定和温室盆栽防病试验。结果表明 ,菌株初步鉴定为芽胞杆菌 (Bacillusspp .)和假单胞杆菌 (Pseudomonasspp .)。其代谢产物蛋白质粗提物对青枯菌的生长有明显抑制作用。温室控病效果在 4 5 0 6 %～ 82 35 % ,可用于有效地控制马铃薯青枯病的发生危害 ,具有广阔的应用前景。
A total of 30 bacterial isolates were isolated from rhizosphere soil of various plants. Nearly 40 strains of X2, B3, 1J ’and 2e’ were screened out from the bacterial culture plates. The results showed that they were more resistant to bacterial wilt (potato and tobacco) Strong antagonism, indoor determination of pathogenic bacteria inhibition zone of 0 8 ~ 30cm. Select 1j ’, 2e’, X2 and B3 for further antibacterial mechanism, strain identification and greenhouse pot disease prevention test. The results showed that the strains were identified as Bacillusspp. And Pseudomonas sp. Crude extracts of their metabolites have a significant inhibitory effect on the growth of R. solanacearum. The control effect of greenhouse is 45.6% ~ 82.35%, which can be used to control the occurrence of potato bacterial wilt effectively and has a broad application prospect.