论文部分内容阅读
目的从钙离子-钙调蛋白依赖性蛋白激酶II(CaMKⅡ)表达等方面探讨不同浓度铅接触与行为记忆改变的关系。方法 32只大鼠随机分为4组:空白组8只,饮双蒸水,模型组24只,分为低、中、高、剂量组,分别按2.4、4.8、9.6 mmol/L的浓度在饮水中添加醋酸铅,60 d后,分别将各组动物进行Morris水迷宫试验;然后收集全血、脑组织原子吸收光谱石墨炉法检测铅含量;采用RT-PCR法和免疫组织化学方法检测脑组织CaMKⅡmRNA及其蛋白表达。结果与空白组比较,各模型组血铅、脑组织铅含量显著增高(P<0.05);Morris水迷宫试验提示模型组大鼠空间定位航行能力下降(P<0.05),铅浓度越高下降越明显(P<0.05);模型组CaMKⅡmRNA及其蛋白表达显著降低(P<0.05)。结论铅能引起铅接触大鼠的行为记忆障碍,且铅接触浓度越高越明显,其机制可能与铅能降低海马区CaMKⅡmRNA及其蛋白依赖性表达等有关。
Objective To investigate the relationship between different concentrations of lead exposure and behavioral memory changes from the expression of calcium ion-calmodulin-dependent protein kinase II (CaMKⅡ). Methods Thirty-two rats were randomly divided into 4 groups: 8 in blank group, double distilled water, and 24 in model group. The rats were divided into low, medium and high dose groups, After 60 days, the rats in each group were subjected to Morris water maze test. Whole blood was collected for detection of lead in brain by graphite furnace furnace atomic absorption spectrometry. The brain tissues were detected by RT-PCR and immunohistochemistry CaMKII mRNA and its protein expression. Results Compared with the blank group, the content of lead in blood lead and brain tissue in each model group was significantly increased (P <0.05). The Morris water maze test showed that the space navigation ability of model group decreased (P <0.05) (P <0.05). The expression of CaMKⅡmRNA and its protein in model group were significantly decreased (P <0.05). Conclusion Lead can cause behavioral impairment in rats exposed to lead, and the higher the lead exposure, the more obvious the mechanism may be related to the decrease of CaMKⅡmRNA and its protein-dependent expression in hippocampus.