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Two new eremophilane sesquiterpene lactones, lβ-angeloyloxy-6β, 10βdihydroxy-8β-methoxyeremophila-7(11)en-8α,12-olide and 1β-angeloyloxy-6β,10α-dihydroxy-8α-methoxyeremo-phila-7(11)en-8β,12-olide were isolated from the extract of the whole plant of Ligularia myriocephala Ling. Their structures and stereochemistry were elucidated by various spectroscopic methods including intensive 2D-NMR techniques (COSY, gHMQC, gHMBC and 1H-1H NOESY) and HR-ESIMS.