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目的检测claudin-5在非小细胞肺癌活检和术后切除标本中的表达及意义。方法采用免疫组化方法分别检测claudin-5、TTF-1、CK5/6及p63在169例活检标本(鳞状细胞癌105例,腺癌64例)及32例手术切除标本中的表达,计算各个指标的敏感度、特异度、阳性预测值及阴性预测值。结果 claudin-5、TTF-1在腺癌中的阳性率均高于鳞状细胞癌,而CK5/6、p63在鳞状细胞癌中阳性率均高于腺癌,4项指标在活检与术后切除标本中的阳性率比较均无差异(P>0.05)。敏感度亦无显著差异(P>0.05),claudin-5、TTF-1和CK5/6特异度高于p63(P<0.05)。结论 claudin-5、TTF-1、CK5/6和p63在非小细胞肺癌诊断中具有重要价值,联合应用有助于鉴别鳞状细胞癌和腺癌。
Objective To detect the expression and significance of claudin-5 in biopsies and resected specimens of non-small cell lung cancer. Methods The expressions of claudin-5, TTF-1, CK5 / 6 and p63 in 169 biopsies (105 cases of squamous cell carcinoma, 64 cases of adenocarcinoma) and 32 cases of resected specimens were detected by immunohistochemistry The sensitivity, specificity, positive predictive value and negative predictive value of each index. Results The positive rates of claudin-5 and TTF-1 in adenocarcinoma were higher than those in squamous cell carcinoma, while the positive rates of CK5 / 6 and p63 in squamous cell carcinoma were higher than those in adenocarcinoma. The four indexes were higher in biopsy and surgery There was no difference in the positive rate of resected specimens (P> 0.05). The sensitivity of claudin-5, TTF-1 and CK5 / 6 were also significantly higher than that of p63 (P <0.05). Conclusions claudin-5, TTF-1, CK5 / 6 and p63 are of great value in the diagnosis of non-small cell lung cancer. Combined use of claudin-5, TTF-1, CK5 / 6 and p63 is helpful to differentiate squamous cell carcinoma from adenocarcinoma.