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为了解周氏啮小蜂的嗅觉分子机制,从其触角中分离出一种气味结合蛋白I,根据转录数据得到该蛋白的基因CcOBP1,通过构建系统发育树和分析密码子偏好性等研究该基因的进化关系.结果表明:周氏啮小蜂的CcOBP1基因长度为378 bp,编码125个氨基酸,预测蛋白含有信号肽,位于第17位和第18位氨基酸之间.CcOBP1 22条同源基因的平均Ka/Ks值为0.499 781,主要接受负选择.基于系统发育树的结果可知,周氏啮小蜂的CcOBP1蛋白与一些寄主广泛的寄生蜂(丽蝇蛹集金小蜂和多胚跳小蜂)的气味结合蛋白亲缘关系密切,与寄主单一的对叶榕传粉榕小蜂亲缘关系较远.比较周氏啮小蜂与10种昆虫CcOBP1基因11条序列密码子的偏好性和同义分异,发现周氏啮小蜂的G+C含量在第3位密码子和编码区较高,即周氏啮小蜂CcOBP1基因的密码子偏好性较高,同义分异也较高.
In order to understand the molecular mechanism of olfactory response in Schizonts beetle, an odorant binding protein I was isolated from its antennae. The gene CcOBP1 was obtained from the transcriptional data. The gene was studied by constructing phylogenetic tree and analyzing codon bias The results showed that the length of CcOBP1 gene was 378 bp and encoded a protein of 125 amino acids and the predicted protein contained the signal peptide located between the 17th and the 18th amino acids.The 22 homologous genes of CocOBP1 The average Ka / Ks value was 0.499781, which mainly accepted negative selection.Based on the results of phylogenetic tree, we found that the CcOBP1 protein of the Schizochytrium copepod and some host parasitic wasps ) Were closely related to the odorant binding proteins of the host and distantly related to the single host of the Ficus microcarpa.The study compared the preference and synonymous differences between the sequences of 11 sequences of the CcOBP1 gene from 10 species of insects, It was found that the G + C content of the Zebravenidae was high at codon 3 and coding region, that is, the codon preference of CcOBP1 gene was higher and the synonymous difference was higher.