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目的血管生成在肿瘤生长和转移中起重要作用。VEGF和Angiopoietin是目前已知特异性作用血管内皮细胞的促血管生成因子。本研究采用RNA酶保护性分析和免疫组织化学的方法对30例食管癌高发区食管癌的癌组织和癌旁组织VEGF,An-giopoietin及其受体的mRNA和蛋白表达水平进行检测。食管癌组织中VEGF及其受体Flt-1,淋巴内皮特异性受体Flt-4,Angiopoietin受体Tie-2以及血管内皮标志物CD31和CD105mRNA较其癌旁组织显著升高(P<0.05),Angiopoietin-1无显著变化。VEGF和An-giopoietin-1分别与其受体Flt-1和Tie-2mRNA上调具有显著的正相关(r分别为0.54和0.71,P<0.05),与CD31和CD105mRNA也具有显著的正相关(P<0.05)。免疫组织化学显示VEGF和Angiopoietin-2在食管癌组织中过度表达,提示食管癌组织中存在血管内皮和淋巴内皮的活跃增殖。联合抑制VEGF,Angiopoietin和淋巴内皮生长因子或其受体可能抑制肿瘤新生血管和淋巴管的形成,从而降低淋巴及血行转移的潜能。
Purpose Angiogenesis plays an important role in tumor growth and metastasis. VEGF and Angiopoietin are currently known as specific pro-angiogenic factors for vascular endothelial cells. In this study, we detected the mRNA and protein levels of VEGF, An-giopoietin and their receptors in 30 esophageal cancer esophageal cancer tissues and adjacent tissues by RNase protection assay and immunohistochemistry. The expressions of VEGF and its receptor Flt-1, lymphatic endothelial specific receptor Flt-4, Angiopoietin receptor Tie-2 and vascular endothelial markers CD31 and CD105 mRNA in esophageal cancer tissues were significantly higher than those in paracancerous tissues (P <0.05) , Angiopoietin-1 no significant change. There was a significant positive correlation between VEGF and An-giopoietin-1 and the upregulation of Flt-1 and Tie-2 mRNA (r = 0.54 and 0.71 respectively, P <0.05) 0.05). Immunohistochemistry showed that VEGF and Angiopoietin-2 overexpression in esophageal cancer tissue, suggesting the existence of active proliferation of vascular endothelial and lymphatic endothelium in esophageal cancer tissue. Combined inhibition of VEGF, Angiopoietin and lymphatic endothelial growth factor or its receptor may inhibit tumor neovascularization and lymphangiogenesis, thereby reducing the potential for lymphatic and hematogenous metastasis.