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目的探讨杏仁中央核内μ阿片受体是否参与大鼠蔗糖溶液摄入的调节及其可能的机制。方法杏仁中央核内注入μ阿片受体激动剂DAMGO或生理盐水,测量大鼠在双瓶选择试验中对蔗糖溶液及蒸馏水的摄入量;利用荧光免疫组织化学方法,在大鼠摄入蔗糖溶液或蒸馏水后,观察杏仁中央核内μ阿片受体/Fos免疫阳性双标记神经元的数量。结果与生理盐水注射组相比,杏仁中央核内注入DAMGO增加了大鼠3 h内的蔗糖溶液摄入量;与蒸馏水摄入组相比,大鼠摄入蔗糖溶液后,杏仁中央核内c-Fos阳性神经元及μ阿片受体/Fos共表达神经元均显著增加(P<0.05)。结论杏仁中央核参与大鼠蔗糖溶液摄入的调节,该调节作用可能部分是由μ阿片受体介导的。
Objective To investigate the regulation of μ opioid receptor in central almond nucleus and the possible mechanism of its involvement in sucrose solution intake in rats. Methods μ-opioid receptor agonist DAMGO or saline was injected into the central nucleus of almonds to measure the intake of sucrose solution and distilled water in the two-bottle selection test. Fluorescent immunohistochemistry was used to detect the intake of sucrose solution Or distilled water to observe the number of μ opioid receptor / Fos immunopositive double labeled neurons in central almond nucleus. Results Compared with the saline injection group, the injection of DAMGO into the central nucleus of the almond increased the intake of sucrose solution within 3 h. Compared with the distilled water intake group, after the rats ingested sucrose solution, the c -Fos positive neurons and mu opioid receptor / Fos co-expression neurons were significantly increased (P <0.05). Conclusion Almond central nucleus participates in the regulation of sucrose solution intake in rats, which may be partly mediated by mu opioid receptor.