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目的了解高效抗反转录病毒联合疗法(highly active antiretroviral the rapy,HAART)早期AIDS患者CD4+CD25+Foxp3+调节性T细胞(regulatory Tcell,Treg)的变化规律,探讨抗病毒治疗过程中Treg细胞的作用。方法前瞻性分析21例接受HAART规律治疗的AIDS患者,收集患者服药前及服药第2、4、8、12周时的静脉血,采用流式细胞仪胞内染色技术检测CD4+CD25+Foxp3+调节性T细胞的水平,分析Treg细胞在治疗过程中的变化规律,以及Treg细胞的变化与免疫活化间的相关性。结果 HAART治疗开始,Treg细胞计数呈逐渐上升的趋势,前4周呈现快速上升,而后上升速度减慢,Treg百分比变化也有相似的规律;CD8+CD38+表达水平随治疗时间延长而下降,未治疗时Treg细胞计数与免疫活化水平呈负相关,治疗后Treg细胞计数4周内的变化与12周CD8+CD38+水平呈负相关,即治疗开始后,前4周内Treg细胞增加越明显,治疗3个月时免疫活化水平下降越明显。结论抗病毒治疗过程中,活化CD8+淋巴细胞比例的下降与外周血中Treg细胞数的增加相关。Treg细胞数量恢复越早,活化CD8+淋巴细胞比例下降越明显。
Objective To investigate the changes of CD4 + CD25 + Foxp3 + regulatory T cells (Tregs) in early active AIDS patients with highly active antiretroviral the rapy (HAART) and to explore the role of Treg cells in anti-retroviral therapy effect. Methods 21 cases of AIDS patients undergoing HAART regular treatment were prospectively studied. Venous blood samples were collected before treatment and at the 2nd, 4th, 8th and 12th week after treatment. The levels of CD4 + CD25 + Foxp3 + were detected by flow cytometry Treg cells in order to analyze the regularity of Treg cells in the course of treatment and the correlation between the changes of Treg cells and immune activation. Results At the beginning of HAART treatment, Treg cell counts showed a gradual upward trend, showing a rapid increase in the first 4 weeks and then a slowing rate of increase. The percentages of Tregs also had similar rules. The expression level of CD8 + CD38 decreased with the prolongation of treatment time. The Treg cell count was negatively correlated with the level of immune activation. The change of Treg cell count within 4 weeks was negatively correlated with the level of CD8 + CD38 + at 12 weeks after treatment, that is, the increase of Treg cells was more obvious in the first 4 weeks after treatment, Month when the level of immune activation decreased the more obvious. Conclusion The decrease of the proportion of activated CD8 + lymphocytes in antiviral therapy is related to the increase of Treg cells in peripheral blood. The earlier the number of Treg cells recovered, the more obvious the proportion of activated CD8 + lymphocytes decreased.