将小鼠粒细胞-巨噬细胞集落刺激因子(GM-CSF)基因转移至肿瘤细胞内可以大大增加肿瘤细胞的免疫原性,有效诱导肿瘤细胞特异性的抗肿瘤免疫,但是该作用不够强大,对已建立的肿瘤作用较弱.而自杀基因治疗虽然可以消除肿瘤负荷,但是不能有效地诱导抗肿瘤免疫应答.本研究以腺病毒作用载体,将小鼠GM-CSF基因与大肠杆菌胞嘧啶脱氨酶基因(CD)联合转移,在体内可以观察到显著的抗肿瘤作用.小鼠体内接种黑色素瘤B16F10细胞后3天,肿瘤局部注射表达GM-CSF的腺病毒载体Ad-GM-CSF和表达CD基因 Transfer of mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) gene into tumor cells can greatly increase the immunogenicity of tumor cells and effectively induce tumor cell specific anti-tumor immunity, but this effect is not powerful enough. The effect on established tumors is weak. Although suicide gene therapy can eliminate tumor burden, it can not effectively induce anti-tumor immune responses. In this study, the adenoviral vector was used to remove mouse GM-CSF gene and cytosine from E. coli. Ammonia gene (CD) combined transfer can be observed in the body significantly anti-tumor effect. 3 days after inoculation of melanoma B16F10 cells in mice, the tumor local injection of GM-CSF expression of adenovirus vector Ad-GM-CSF and expression CD gene