论文部分内容阅读
目的探讨CEA重组痘苗病毒(rV-CEA)治疗CEA＋肿瘤的机制。方法以我室构建的rV-CEA, 腹腔接种供体C57/BL小鼠，取其脾细胞及腹腔巨噬细胞(MΦ)，分别过继转移给荷CEA＋-HePa肝癌细胞的C57/BL小鼠，检测该供体小鼠脾细胞、腹腔MΦ及相应受体的脾细胞体外杀瘤细胞的效应。结果接种rV-CEA的供体小鼠的脾细胞及腹腔MΦ过继免疫给受体小鼠，具有明显抑制受体CEA阳性肿瘤生长的作用。体外实验表明，该供体脾细胞及接种了供体MΦ的受体脾细胞对同一靶细胞的杀伤活性明显增强，但供体MΦ体外的细胞毒活性无明显增加。结论rV-CEA对CEA＋肿瘤的抑制作用，可能主要通过CEA特异性免疫反应激活T细胞而实现。MΦ作为抗原提呈细胞可通过激活T细胞而杀伤肿瘤细胞，具体机制值得进一步研究。
Objective To investigate the mechanism of CEA+ tumors treated with CEA recombinant vaccinia virus (rV-CEA). Methods C57/BL mice were inoculated intraperitoneally with rV-CEA constructed in our department. The spleen cells and peritoneal macrophages (MΦ) were taken from the mice and they were transferred to C57/BL mice bearing CEA+-HePa hepatoma cells, respectively. The donor mouse spleen cells, peritoneal MΦ and splenocytes of the corresponding recipients were tested for the effect of killer cells in vitro. RESULTS: Spleen cells from donor mice inoculated with rV-CEA and adopting MΦ in the peritoneal cavity were adoptively immunized to recipient mice, which had a significant inhibitory effect on the growth of receptor CEA-positive tumors. In vitro experiments showed that the donor spleen cells and recipient spleen cells inoculated with the donor MΦ significantly increased the killing activity against the same target cells, but the cytotoxic activity of the donor MΦ did not increase significantly. Conclusion The inhibitory effect of rV-CEA on CEA+ tumors may be achieved mainly through activation of T cells by CEA-specific immune responses. MΦ as an antigen-presenting cell can kill tumor cells by activating T cells, and the mechanism is worthy of further study.