论文部分内容阅读
目的观察血管性血友病因子(vWF)及其裂解酶ADAMTS13在慢性肾脏病不同病理类型中的表达以及与临床指标的相关性。方法采用免疫组织化学分析vWF和ADAMTS13在36例不同病理类型慢性肾炎患者肾脏中的表达,Image J图象分析软件测定vWF和ADAMTS13的阳性染色面积的比例。结果 (1)肾病综合征患者肾小球阳性染色面积vWF/ADAMTS13明显高于慢性肾炎组(P<0.05);(2)膜性肾病组ADAMTS13表达明显低于非膜性肾病组(P<0.05);(3)vWF/ADAMTS13阳性染色面积比与蛋白尿呈正相关(r=0.512,P<0.01)。结论慢性肾脏病局部vWF和ADAMTS13表达间平衡失调与慢性肾脏病的临床和病理类型有关,能在一定程度上反映肾脏微循环的变化。
Objective To observe the expression of von Willebrand factor (vWF) and its lytic enzyme ADAMTS13 in different pathological types of chronic kidney disease (CKD) and its correlation with clinical parameters. Methods The expression of vWF and ADAMTS13 in the kidney of 36 patients with chronic glomerulonephritis with different pathological types was analyzed by immunohistochemistry. The proportion of positive staining area between vWF and ADAMTS13 was determined by Image J image analysis software. Results (1) The area of glomerular positive staining vWF / ADAMTS13 in patients with nephrotic syndrome was significantly higher than that in chronic nephritis group (P <0.05). (2) The expression of ADAMTS13 in membranous nephropathy group was significantly lower than that in non-membranous nephropathy group ). (3) The positive staining area ratio of vWF / ADAMTS13 positively correlated with proteinuria (r = 0.512, P <0.01). Conclusion The imbalance between vWF expression and ADAMTS13 expression in chronic kidney disease is related to the clinical and pathological types of chronic kidney disease, which can reflect the changes of renal microcirculation to a certain extent.