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目的 探讨王氏管精子优选法在精子优选的应用 ,并与传统的精子上游法进行比较。方法 随机选择 5 7份精液 ,采用两种方法进行配对处理 ,比较两种方法分离前后、两者间精子活动率、形态以及精子顶体形态和精子染色质等参数的变化。结果 两种方法处理后的精子活动率、精子形态差异有显著性 (P <0 0 1) ,王氏管法处理后精子顶体完整率、正常染色质率亦显示出显著的差异 (P <0 0 1)。结论 王氏管精子优选法和精子上游法在精子处理中获得较好的精子质量 ,而王氏管精子优选法则获得更优质的精子 ,且具有方便操作实用的特点
Objective To investigate the application of spermatozoa in spermatozoa in sperm selection and to compare with the traditional sperm upstream method. Methods A total of 57 semen samples were randomly selected and matched by two methods. The changes of sperm motility, morphology, acrosome morphology and sperm chromatin were compared before and after separation. Results There was a significant difference in sperm motility and sperm morphology between the two treatments (P <0.01). There was also a significant difference in sperm acrosome integrity and normal chromatin after treatment (P < 0 0 1). Conclusion Wang sperm sperm optimization method and sperm upstream method in sperm processing to obtain better sperm quality, and Wang tube sperm optimization rules to obtain better sperm, and has the characteristics of easy to operate and practical