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目的观察央芪汤流浸膏制剂对胃癌种植瘤瘤体凋亡作用的影响。方法将人胃癌SGC-7901细胞悬液皮下接种于裸鼠右侧背部皮下,当接种部位肿瘤长至最大径4-5mm时,裸鼠随机分为4组:央芪汤敷贴剂组;氟尿嘧啶组;联合用药组和生理盐水对照组。给药期间观察裸鼠的一般情况及移植瘤的生长状况,使用游标卡尺测量和记录肿瘤的最大径(a)最小径(b),绘制肿瘤体积的生长曲线;连续用药24 d,并继续观察4d后,颈椎脱位处死裸鼠,连同胞膜完整剥出肿瘤组织,电子天平称重;按体积计算药物的抑瘤率;采用Tunel法检测细胞凋亡。结果央芪汤流浸膏可以明显增加荷瘤裸鼠肿瘤细胞凋亡,凋亡指数与氟尿嘧啶组和对照组相比,有明显差异(P<0.01)。结论央芪汤流浸膏可以通过促进胃癌细胞的凋亡达到抗肿瘤的目的。
Objective To observe the effects of the extract of Yangqi Tangliu on the apoptosis of tumor-bearing tumor of gastric cancer. Methods Human gastric cancer cell line SGC-7901 was subcutaneously inoculated subcutaneously in the right back of nude mice. When the inoculation site grew to a maximum diameter of 4-5 mm, the nude mice were randomly divided into 4 groups: Group; combination group and saline control group. During the administration, the general situation of the nude mice and the growth of the tumor were observed. The maximum diameter (a) and the minimum diameter (b) of the tumor were measured and recorded using a vernier caliper, and the growth curve of the tumor volume was drawn. After the cervical spine dislocation, the nude mice were sacrificed, and the tumor tissue was completely stripped with the cell membrane. The electronic balance was weighed. The inhibition rate of the drug was calculated by volume. The apoptosis was detected by Tunel method. Results Yangqi decoction could significantly increase the apoptosis of tumor cells in nude mice bearing tumor. The apoptotic index was significantly different from that of the control group (P <0.01). Conclusion Yangqi Tangliu extract can promote the apoptosis of gastric cancer cells to achieve the purpose of anti-tumor.