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随着高分子科学研究的不断发展,凝胶渗透色谱(GPC)作为测定高聚物分子量及分子量分布的方法已被普遍采用。 CPC方法的心脏部分是GPC色谱柱。柱效的高低直接影响检测结果的准确性。选择长度-直径比合适的柱子和孔径、孔容及粒度符合要求的载体,是保证柱效的重要因素。满足这些因素之后,装柱的好坏,则是直接影响柱效的关键。文献上有关GPC装柱方法的报导不多;所报导的简易GPC的理论塔板数一般为400—1500/米;在测试过程中有加宽现象,处理数据时一般需要进行较为繁琐的加宽校正。
With the continuous development of polymer science, gel permeation chromatography (GPC) has been widely used as a method to determine the molecular weight and molecular weight distribution of polymers. The heart of the CPC method is a GPC column. The level of efficiency directly affects the accuracy of test results. Choosing a carrier with a length-to-diameter ratio that matches the desired column and aperture, pore volume, and particle size is an important factor in ensuring efficiency. Satisfying these factors, loading column is good or bad, it is the key to directly affect the efficiency. The literature on the method of GPC packing is poorly reported; the number of theoretical plates reported for simple GPCs is typically 400-1500 / m; there is widening in the course of the test, generally requiring more tedious broadening Correction.