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目的探讨狂犬病病毒G蛋白基因重组及其对病毒属性的影响。方法收集GenBank狂犬病病毒G蛋白基因全序列,经过MEGA4.1对齐、去除空位处理后,运用RDP 2.0软件进行筛查,然后利用SimPlot 3.5.1软件进行相似性分析和BOOTSCAN分析,对基因重组加以验证,并构建最大似然进化树,观察重组片断拓扑结构的改变。结果利用RDP 2.0软件共检测到3个重组序列,分别是DQ849069、AF334789与AY009100。相似性分析和BOOTSCAN分析确认重组序列DQ849069重组特征明显,重组片段为746～1 566nt,重组片断最大似然进化树的拓扑结构也发生了相应改变。而AF334789与AY009100未发现有意义重组信号;地缘分析表明DQ849069是基因重组的产物。结论狂犬病病毒G蛋白在进化过程中存在重组,重组事件对未来病毒毒力的影响有待于深入研究。
Objective To investigate the recombination of rabies virus G protein gene and its effect on virus properties. Methods The whole sequence of G protein gene of GenBank rabies virus was collected and aligned with MEGA4.1. After removing the vacancies, RDP 2.0 software was used for screening. Then the similarity analysis and BOOTSCAN analysis were performed by SimPlot 3.5.1 software to verify the gene recombination , And construct the maximum likelihood evolutionary tree to observe the change of the topological structure of the recombinant fragment. Results Three recombinant sequences were detected using RDP 2.0 software, namely DQ849069, AF334789 and AY009100. Similarity analysis and BOOTSCAN analysis confirmed that the recombination sequence of the recombinant sequence DQ849069 was obvious, with the recombinant fragment of 746-1 566nt. The topological structure of the maximum likelihood tree of recombinant fragment also changed accordingly. However, no significant recombination signal was found between AF334789 and AY009100. Geoanalysis showed that DQ849069 was the product of gene recombination. Conclusion There is recombination in rabies virus G protein evolution and the impact of recombination events on future virulence needs to be further studied.