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目的:建立同时测定心通口服液中葛根素、毛蕊异黄酮葡萄糖苷、柚皮苷及淫羊藿苷含量的方法。方法:采用Kromasil C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-水为流动相,梯度洗脱,流速1 mL.min-1,柱温30℃,检测波长λ1=250 nm(检测葛根素),λ2=260 nm(检测毛蕊异黄酮葡萄糖苷),λ3=283 nm(检测柚皮苷),λ4=270 nm(检测淫羊藿苷)。结果:葛根素、毛蕊异黄酮葡萄糖苷、柚皮苷及淫羊藿苷的线性范围分别为0.441～11.0,0.031 2～0.780,0.186～4.66,0.134～3.47μg,平均加样回收率分别为98.53%,98.03%,98.64%,99.16%。结论:方法操作简便,快速,重复性好,可作为该产品质量控制的方法。
Objective: To establish a method for simultaneous determination of puerarin, calycosin glucoside, naringin and icariin in Xintong Oral Liquid. Methods: A Kromasil C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase consisted of acetonitrile and water with a gradient of 1 mL · min-1 and the column temperature was 30 ℃. The detection wavelength was λ1 = 250 nm Puerarin), λ2 = 260 nm (detection of calycosin glucoside), λ3 = 283 nm (detection of naringin), λ4 = 270 nm (detection of icariin). Results: The linear ranges of puerarin, calycosin glucoside, naringin and icariin were 0.441 ~ 11.0,0.031 2 ~ 0.780,0.186 ~ 4.66,0.134 ~ 3.47μg, the average recoveries were 98.53% , 98.03%, 98.64%, 99.16%. Conclusion: The method is simple, rapid, reproducible and can be used as a method of product quality control.