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目的:了解左旋咪唑(LMS)不同阶段给药对实验性变态反应性脑脊髓炎(EAE)大鼠中枢神经系统(CNS)内MCP-1、MIP-1α表达的影响。方法:采用豚鼠脊髓匀浆免疫Wistar大鼠建立EAE模型,分别在免疫前(LMS1)、免疫同时(LMS2)、免疫后(LMS3)给予LMS10mg·kg-1,采用行为学变化观察发病情况,常规苏木精-伊红和Kluver&Barrera髓鞘染色法观察CNS病理变化,免疫组化法及原位杂交法观察MCP-1、MIP-1α的表达。结果:LMS1及LMS2组能加重行为学及病理变化(P<0.01,P<0.05),而LMS3组出现复发。免疫组化和原位杂交结果:和EAE组相比,LMS1,LMS2组MCP-1、MIP-1α的表达量明显升高(P<0.01,P<0.05)。结论:LMS能明显增加EAE大鼠CNS内MCP-1、MIP-1α的表达。提示LMS有促进EAE炎性细胞的趋化、激活作用,在免疫反应炎症细胞向CNS迁移过程中起重要的作用。
Objective: To investigate the effects of levamisole (LMS) at different stages on the expression of MCP-1 and MIP-1α in the central nervous system (CNS) of experimental allergic encephalomyelitis (EAE) rats. Methods: Wistar rats were immunized with guinea pig spinal cord homogenate to establish EAE model. LMS1, LMS2 and LMS3 were given to LMS 10 mg · kg-1, respectively. The changes of behavioral changes were observed. The pathological changes of CNS were observed by hematoxylin-eosin and Kluver & Barrera myelin staining. The expressions of MCP-1 and MIP-1α were observed by immunohistochemistry and in situ hybridization. Results: LMS1 and LMS2 groups increased behavioral and pathological changes (P <0.01, P <0.05), while relapse occurred in LMS3 group. Immunohistochemistry and in situ hybridization results: Compared with EAE group, the expression of MCP-1 and MIP-1α in LMS1 and LMS2 groups were significantly increased (P <0.01, P <0.05). Conclusion: LMS can significantly increase the expression of MCP-1 and MIP-1α in the CNS of EAE rats. It is suggested that LMS can promote the chemotaxis and activation of inflammatory cells in EAE and play an important role in the migration of inflammatory cells to CNS.