论文部分内容阅读
放射免疫测定,灵敏度高,特异性强。但在实验中产生误差的原因是错综复杂的,多种多样的,常见于以下几种: 1 系统误差(定向误差或恒定误差) 系统误差指实验中,比较恒定的原因引起的。造成误差的原因有:(1)属于实验内的原因:①仪器误差:如γ计数器、离心机、加样器、冰箱等。②试剂误差:试剂盒中Ag的比放射性和免疫活性。Ab的特异性,标准品的纯度及量的大小,合适的缓冲体系,分离试剂等。③操作误差:实验操作中,由于实验人员所掌握的操作规程和实验条件的不同而引起的。如加样的习惯、快慢等。④方法误差:不同厂家的药盒测定值不同。如TSH、TGA,北方偏高,南方偏低。(2)属于实验外部原因:主要有外界的温度、电流、电压等对仪器性能的改变。如T_3、T_4当炎热的夏天,温度升高,γ计数器不稳造成值偏高。
Radioimmunoassay, high sensitivity and specificity. However, the reasons for the error in the experiment is complex, varied, common in the following categories: A systematic error (directional error or constant error) System error refers to the experiment, the more constant causes. The reasons for the error are: (1) belong to the experimental reasons: ① instrument error: such as γ counter, centrifuge, sample changers, refrigerators and so on. ② reagent error: Ag than the radioactivity and immunological activity of the kit. Ab specificity, purity and amount of standard size, the appropriate buffer system, separation reagents. ③ operating error: experimental operation, due to experimental staff to grasp the operating procedures and experimental conditions caused by different. Such as adding kind of habit, speed and so on. ④ method error: Different manufacturers of the kit measured values are different. Such as TSH, TGA, high in the north, low in the south. (2) belong to the experimental external reasons: the main external temperature, current, voltage, etc. on the instrument performance changes. Such as T_3, T_4 when the hot summer, the temperature increases, γ counter instability caused by high value.