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采用DAPI染色和红血球计数板计数分析了艾比根土壤DNA提取试剂盒的DNA提取效率,以及钢珠研磨和酶处理对DNA提取效率的影响.结果表明,艾比根土壤DNA提取试剂盒不能完全破碎土壤微生物细胞,DNA提取效率还有提升的空间.采用钢珠研磨、溶菌酶和蛋白酶处理能显著提高艾比根试剂盒DNA的提取效率,改进后的方法可以较有效的用于提取土壤和活性污泥中微生物群落的DNA.
The DNA extraction efficiency of Abidjan soil DNA extraction kit and the effect of steel ball grinding and enzyme treatment on the DNA extraction efficiency were analyzed by DAPI staining and erythrocytic plate counting.The results showed that the Abidjan soil DNA extraction kit can not be completely broken Soil microbial cells, DNA extraction efficiency and room for improvement.With ball milling, lysozyme and protease treatment can significantly improve the DNA extraction efficiency of the ABI kit, the improved method can be more effective for the extraction of soil and activated sludge Microbial community DNA in the mud.