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本文用荧光素标记麦芽凝集素(WGA)的方法,研究埃及伊蚊血细胞表面的变化与在胸腔内接种犬恶丝虫微丝蚴后产生的黑变反应。埃及伊蚊利物浦黑眼株饲养在模拟环境中,温度26.5±1℃、温度75±10％,光照周期为16小时光照,8小时黑暗,成蚊喂以0.3M蔗糖。以单个雌蚊灌注20μl Earle’s平衡盐水溶液至血腔,然后收集灌洗液。将灌洗液放在载玻片上5～6分钟,血细胞沉淀后用2滴冷(4℃)丙酮固定,用蒸馏水轻洗粘附
In this paper, we investigated the changes of the blood cell surface of Aedes aegypti mosquitoes by using fluorescein-labeled maltogenic lectin (WGA) and the blackening reaction after inoculation of microfilaria filariasis in the thoracic cavity. The Aedes aegypti Liverpool black-eye strain was housed in a simulated environment at a temperature of 26.5 ± 1 ° C and a temperature of 75 ± 10% with a light period of 16 hours of light and an 8-hour darkness and an adult mosquito fed 0.3 M sucrose. A single female mosquito was infused with 20 [mu] l of Earle’s Balanced Saline solution into the blood chamber before collecting the lavage fluid. Place the lavage fluid on a glass slide for 5 to 6 minutes, fix with 2 drops of cold (4 ° C) acetone after precipitation of the blood cells, wash gently with distilled water