论文部分内容阅读
目的 探讨神经节细胞 (RGC)在实验性视网膜脱离及复位状态下的反应 ,检测白介素 - 1β(IL - 1β)、白介素 - 1受体拮抗物 (IL - 1Ra)在 RGC丢失过程中的作用。 方法 73只 SD大鼠用 1%荧光金(FG)经上丘、外侧膝状体逆行标记 ,healon GV(1.4 %透明质酸钠 )鼻侧视网膜下注射。 10 ng IL - 1Ra和5 0 0 ng IL - 1β抗体随 healon GV一同注入视网膜下。对逆行标记后 10 d,视网膜脱离后 2 h、1、3、5、7、10、2 0、5 0、90 d,视网膜脱离 10 d复位 30 d,视网膜脱离 90 d复位 2 0 d,IL - 1Ra和 IL - 1β抗体视网膜下注入后 1d和 10 d取材的视网膜进行铺片 ,在荧光显微镜下拍片 ,进行 RGC计数。对照组则在球内注入相应计量healon GV。 结果 视网膜脱离后 2 h开始有 RGC的丢失 ,1d时达到高峰 ,然后缓慢下降。在未脱离区 ,亦有 RGC的丢失。视网膜脱离 10 d后复位 (早期复位 )可停止 RGC的丢失 ,视网膜脱离 90 d后复位 (晚期复位 )可使 RGC进一步丢失 ,最后停留在某一水平。IL- 1β抗体、IL- 1Ra可以减少这种丢失。 结论 视网膜脱离状态下 ,脱离区与未脱离区都有 RGC的丢失。早期复位可使 RGC的丢失停止 ,晚期复位可进一步损伤 RGC。拮抗 IL- 1β能减少 RGC的丢失 ,IL- 1Ra对 RGC有保护作用。
Objective To investigate the response of ganglion cells (RGC) in experimental retinal detachment and reattachment and to explore the role of interleukin - 1β (IL - 1β) and interleukin - 1 receptor antagonist (IL - 1Ra) in RGC loss. Methods 73 SD rats were retrogradely labeled with 1% fluorescent gold (FG) through the superior colliculus and lateral geniculate body. Healon GV (1.4% sodium hyaluronate) was injected subcutaneously into the nasal cavity. 10 ng IL - 1Ra and 500 ng IL - 1β antibodies were injected into the retina with healon GV. At 10 days after retrograde labeling, retinal detachment at 2 h, 1, 3, 5, 7, 10, 20, 50, 90 d, retinal detachment 10 d, 30 d, The retinas of 1Ra and IL - 1βantibodies drawn 1d and 10d after subretinal injection were plated and counted under a fluorescence microscope for RGC counting. In the control group, correspondingly healon GV was injected into the ball. Results The loss of RGC began 2 hours after retinal detachment, reached the peak on 1 day, then decreased slowly. In the area without leaving, there are also lost RGC. Retinal detachment 10 days after the reset (early reset) to stop the loss of RGC, 90 days after retinal detachment (late reset) can further RGC loss, and finally stay at a certain level. IL-1β antibody, IL-1Ra can reduce this loss. Conclusion In the state of retinal detachment, there is loss of RGC in both the deviated area and the non-detachment area. Early loss of RGC can stop the loss, advanced resection can further damage the RGC. Antagonism of IL-1β can reduce the loss of RGC, IL-1Ra has a protective effect on RGC.