为了探索转基因遗传及染色体重组对转基因表达的影响,以4个转绿色荧光蛋白基因(gfp)大麦系与野生型大麦相互杂交,通过对F2根尖和花粉细胞中绿色荧光蛋白(GFP)的检测,研究了转基因的遗传方式和表达。结果表明,绿色荧光蛋白基因在单位点和双位点插入的转基因系与野生型大麦杂交的F2群体中分别表现3∶1和15∶1的遗传分离,符合孟德尔式遗传,并表现基因的剂量效应;不同转基因系间相互杂交后代同样表现孟德尔式遗传,并出现转基因gfp表达水平提高的个体;转基因沉默系参与的杂交后代继续表现其转基因沉默。 In order to explore the effects of transgenic and chromosomal recombination on transgene expression, four green fluorescent protein (gfp) -transgenic lines were crossed with wild-type barley to detect the expression of green fluorescent protein (GFP) in the apical and pollen cells of F2. , Studied the genetic mode and expression of the transgene. The results showed that the GFP gene showed genetic segregation of 3:1 and 15:1 respectively in F2 populations of transgenic lines inserted at single and double loci and wild-type barley, which met Mendelian inheritance and expressed genes Dose effect. The crosses between different transgenic lines also showed Mendelian inheritance, and individuals with elevated gfp expression appeared. Individuals with transgenic silent lines continued to express their transgenic silence.