纯系Wistar大鼠皮下注射醋酸可的松25mg/次,每周2次,用药7周即可成功诱发大鼠肺孢子虫肺炎。相差显微镜直接镜检、姬姆萨氏染色(Giemsa’s stain)、六亚甲基四胺银染色(GMS stain)和亚甲胺蓝染色(TBO stain)均可检出包囊。其中六亚甲基四胺银染色可显示囊壁特征性的括弧样结构,具有确诊价值。病理改变主要为肺泡间隔增厚和淋巴细胞、单核细胞浸润,肺泡中少量蜂窝状渗出物。对肺孢子虫的滋养体、囊子前期和囊子的超微结构进行了电镜观察 Pure line Wistar rats subcutaneously injected cortisone acetate 25mg / times, twice a week, 7 weeks of treatment can successfully induce pneumocystis pneumonia in rats. Cysts can be detected by direct microscopy with phase contrast microscopy, Giemsa’s stain, GMS stain, and TBO stain. One of the hexamethylenetetramine silver staining can show characteristic cystic wall-like structure, with a definite value. Pathological changes mainly for thickening of alveolar septa and lymphocytes, monocyte infiltration, a small amount of alveolar honeycomb exudate. Pneumocystis trophozoites, cystic pre-and ultrastructural ultrastructure were observed electron microscopy