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转草鱼(Ctenopharyngodon idellus)生长激素基因鲤(Cyprinus carpio)是一种以提高生长速度为目标的鱼类新品种,但是转基因可能改变了转基因鲤对不同环境条件的响应对策。为了探讨投喂水平对快速生长转基因鲤能量分配和越冬存活的影响,本研究在两种投喂水平(饱食和半饱食)下开展了对转基因鲤和对照鲤的生长和越冬实验。在夏秋季生长实验中,饱食投喂转基因鲤(satiation transgenic,ST)的体重生长速度是饱食投喂对照鲤(satiation nontransgenic,SN)的1.33倍,半饱食投喂转基因鲤(half-satiation transgenic,HT)的体重生长速度则是半饱食投喂对照鲤(half-satiation nontransgenic,HN)的1.51倍,但是HT和SN组的体重生长速度没有显著性差异。HN,SN,HT和ST组的水分含量依次减小。同一基因型不同投喂水平下的蛋白含量没有显著性差异,但ST组的蛋白含量显著高于SN组。HN,SN,HT和ST组的脂肪含量依次显著增加。在秋季末期,对照鲤的饱食水平明显下降,而转基因鲤的饱食水平并没有明显下降。各组实验鱼的越冬存活率无显著性差异,投喂史只对越冬后的能量含量有显著性影响。本研究表明,转基因鲤和对照鲤均能对投喂水平和越冬做出一致的能量分配响应,限制投喂能激发转基因鲤优先保障生长能量供应而减少能量的存储,转基因鲤和对照鲤的越冬存活能力没有明显差异。本研究结果可为转基因鲤生态安全评估提供参考。
Ctenopharyngodon idellus Cyprinus carpio is a new species of fish that aims to increase its growth rate. However, transgene may alter the response of transgenic carp to different environmental conditions. In order to investigate the effect of feeding on the energy allocation and winter survival of rapidly growing transgenic common carp, we conducted experiments on the growth and overwintering of transgenic carps and control carps at two different feeding levels (fed and half-full). In summer and autumn growth experiments, the body weight growth rate of satiation transgenic (ST) fed with full-time feeding was 1.33 times higher than that of satiation nontransgenic (SN) The weight growth rate of satiation transgenic mice was 1.51 times that of half-satiation nontransgenic carp (HN), but there was no significant difference in body weight growth rate between HT and SN groups. The water content of HN, SN, HT and ST groups decreased in turn. There was no significant difference in the protein content of the same genotype at different feeding levels, but the protein content of ST group was significantly higher than that of SN group. The fat content of HN, SN, HT and ST groups increased significantly. At the end of autumn, the control group had a significant decrease in the level of satiety of the control carps while the level of satiety of transgenic carp did not decrease significantly. There was no significant difference in the overwintering survival rate of experimental fish in each group, and the feeding history only had a significant effect on the energy content after overwintering. This study shows that transgenic carp and control carp can make a consistent energy allocation response to the level of feeding and overwintering, limiting the feeding can stimulate transgenic carp give priority to protection of growth energy supply and reduce energy storage, transgenic carp and control carp overwintering There was no significant difference in viability. The results of this study may provide a reference for the ecological safety assessment of transgenic carp.