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目的研究流式细胞仪检测儿童急性B淋巴细胞性白血病(B-ALL)免疫分型的临床价值,并探讨与预后的关系。方法利用流式细胞仪对39例B-ALL患儿在初诊时进行免疫分型。CD45-SSC设门,在CD19、CD10、CD20和CD22中选择两个单克隆抗体组合,进行三色组合分析。髓系抗体选用CD33和CD13。根据是否伴有髓系抗原表达分为两组,并随访预后。结果①CD19、CD22、CD10的阳性率都超过或接近90%,分别为CD19:94.87%,CD22:92.31%,CD10:87.18%,而CD20只有15.38%的阳性率;有33.33%的患儿检测到髓系抗原(My)标志,其中以CD1+38例,CD3+35例;②共有6例患儿复发,两组经χ2精确概率法检验,P>0.05,差异无统计学意义。结论①流式细胞仪可以用于绝大多数的B-ALL患儿免疫表型的检测;②伴有髓系表达不具有提示复发的意义。
Objective To investigate the clinical value of flow cytometry in the detection of childhood B-ALL immunophenotype and to explore its relationship with prognosis. Methods 39 cases of B-ALL children were immunophenotyped by flow cytometry. CD45-SSC gated to select two monoclonal antibody combinations on CD19, CD10, CD20 and CD22 for tri-color combinatorial analysis. Myeloid antibody selection CD33 and CD13. According to whether there is associated with myeloid antigen expression is divided into two groups and follow-up prognosis. Results ①The positive rates of CD19, CD22 and CD10 were all higher than or close to 90%, which were respectively CD19: 94.87%, CD22: 92.31%, CD10: 87.18% and CD20 with only 15.38%; 33.33% Myeloid antigen (My) markers, including CD1 + 38 cases, CD3 + 35 cases; ② A total of 6 children relapsed, the two groups by χ2 exact test, P> 0.05, the difference was not statistically significant. Conclusion ① Flow cytometry can be used to detect the immunophenotype in the vast majority of children with B-ALL; ② With myeloid expression does not suggest the significance of recurrence.