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用子叶法接种大豆疫霉根腐病1号生理小种,分析抗病亲本绥农10和感病寄主Williams的杂交F1、F2、F3的抗病性。结果表明,F1单株均表现为抗病;F2群体抗感分离比例符合3∶1;由F2感病单株衍生出的F3株系均表现感病,F2抗病单株衍生出的F3株系抗感分离株系比值符合1∶2;说明绥农10中对大豆疫霉根腐病1号生理小种的抗性受1对单显性基因控制,将该抗病基因拟名为RpsSN10。用500对大豆SSR引物和124株绥农10/Wil-liamsF2分离群体对RpsSN10基因进行定位,将RpsSN10基因定位在F连锁群上,其中标记Satt423和Satt149与RpsSN10基因的遗传距离分别为9.8cM和11.2cM,并分别位于目的基因的两侧。
The inoculation of P. sojae root rot # 1 was carried out by cotyledon method, and the disease resistance of F1, F2 and F3 were analyzed. The results showed that all the F1 plants exhibited resistance to disease, and the resistant population of F2 population conformed to 3:1. The F3 lines derived from F2 susceptible lines all showed susceptible disease. The F3 lines derived from F2 resistant plants The ratio of lines resistant to susceptible lines was 1: 2, indicating that the resistance of Suinong 10 to P. sojae root rot 1 was controlled by a single dominant gene. The resistance gene was named RpsSN10 . 500 pairs of soybean SSR primers and 124 strains of Suinong 10 / Wil-liams F2 were used to locate the RpsSN10 gene. The RpsSN10 gene was located on the F linkage group. The genetic distances of Satt143 and Satt149 to RpsSN10 were 9.8 cM and 11.2cM, respectively, located on both sides of the gene of interest.