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以叶子花‘大红宝巾’(Bougainvillea glabra‘Mrs Butt’)为试材,研究外源脱落酸(ABA)对其开花及其生物合成关键酶含量与活性变化的影响。结果表明:外源ABA对叶子花的成花有促进作用;去甲二氢愈创木酸(NDGA)浓度大于10μmol·L-1时,ABA的合成明显被抑制,低浓度NDGA与高浓度ABA共同作用反而对内源ABA含量的升高具有增效作用;内源ABA的变化趋势与9–顺式–环氧类胡萝卜素双氧合酶(NCED)含量的变化趋势相一致,表明NCED为ABA生物合成途径中的限速酶;外源ABA使玉米黄质环氧化酶(ZEP)、NCED、ABA醛氧化酶(AAO)含量及活性均明显上升,表明ABA能自我催化调节其合成,且单一酶基因或单一酶促反应并不能合理解释ABA水平的变化,推测ABA信号的作用机制是一个由多酶有机统筹系统控制的结果。
Using Bougainvillea glabra’Mrs Butt ’as test material, the effects of exogenous ABA on the content of key enzyme and its activity in flowering and its biosynthesis were studied. The results showed that the exogenous ABA promoted the flowering of the flower of the leaves. The synthesis of ABA was significantly inhibited when the concentration of nordihydroguaiaretic acid (NDGA) was greater than 10 μmol·L-1. The low concentrations of NDGA and high concentrations of ABA But the synergistic effect could have a synergistic effect on the increase of endogenous ABA content. The trend of endogenous ABA was consistent with that of 9-cis-epoxy carotenoid dioxygenase (NCED), indicating that NCED was ABA biosynthetic pathway. Exogenous ABA increased the content and activity of zeaxanthin cyclooxygenase (ZEP), NCED and ABA aldehyde oxidase (AAO), which indicated that ABA could self-catalyze the synthesis of a single Enzyme gene or single enzymatic reaction does not reasonably explain the changes of ABA level. It is speculated that the mechanism of action of ABA signal is a result of multi-enzyme organic integrated system control.