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目的:探索建立一种操作简便、快速、稳定、成功率高,易于推广的羊水细胞原位培养染色体制备方法。方法:选择近四年在本院进行产前诊断的1109例孕妇的羊水,采用改良羊水原位细胞培养染色体制备技术进行产前诊断。结果:缩短培养时间(7～9天收获)及染色体制片时间,细胞丢失少,分裂相多,培养成功率为99.6%。结论:该方法简便、快速、稳定,细胞丢失少,分裂相多,技术要求低,便于基层单位推广应用。
OBJECTIVE: To explore a method for the preparation of chromosomes for in situ culture of amniotic fluid cells which is simple, rapid, stable, successful and easy to popularize. Methods: The amniotic fluid of 1,109 pregnant women who had been diagnosed prenatally in our hospital in the past four years was selected for prenatal diagnosis by modified in situ cell culture of chromosomal preparation of amniotic fluid. Results: Shorten the culture time (7 ~ 9 days harvest) and chromosome preparation time, less cell loss, more split phase, the success rate of culture was 99.6%. Conclusion: The method is simple, rapid, stable, less cell loss, more split phase, low technical requirements, to facilitate the promotion and application of grass-roots units.