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目的:研究CD14基因启动区C(-269)T分子变异与中国人群冠心病的关联。方法:用PCR技术检测了50例经冠脉造影证实的冠心病患者及90例健康人CD14基因启动区含(-269)多态位点的基因片段,HaeⅢ酶切分型。计算基因型及等位基因频率,并比较两组间差异。结果:冠心病组T等位基因(无酶切位点)频率显著高于对照组(51.00％Vs32.78％;OR=2.13;95％ CI,1.30～3.50;P<0.01)。结论:CD14基因启动区C(-269)T分子变异与中国人群冠心病相关。
Objective: To investigate the association between C (-269) T mutation in promoter region of CD14 gene and coronary heart disease in Chinese population. Methods: The gene fragments of (-269) polymorphisms in promoter region of CD14 gene in 50 patients with coronary heart disease confirmed by coronary angiography and 90 healthy people were detected by PCR. Hae Ⅲ digestion was performed. Genotype and allele frequencies were calculated and the differences between the two groups were compared. Results: The frequency of T allele (no cleavage site) in CHD group was significantly higher than that in control group (51.00% Vs 32.78%; OR = 2.13; 95% CI 1.30-3.50; P <0.01). Conclusion: The variation of C (-269) T in promoter region of CD14 gene is associated with coronary heart disease in Chinese population.