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目的:探讨血管内皮生长因子(VEGF)对原发性肝细胞癌(PHC)临床诊断的价值。方法:选择21例PHC及36例肝硬化患者作为研究对象,采用酶联免疫吸附方法检测其血清VEGF表达水平,分析其与甲胎蛋白(AFP)的相关性及对PHC发生的判断价值。结果:PHC患者血清VEGF表达水平高于肝硬化患者,肝硬化患者高于健康对照组(Hc=19.672,P<0.05);血清VEGF与AFP呈正相关(r2=0.488,P<0.05);血清VEGF、AFP预测PHC发生的接受者操作特性曲线(ROC)为0.819和0.774,二者均能预测PHC发生(P<0.05);以VEGF≥106.6 pg/m L和AFP≥30.06 ng/m L作为预测指标,ROC为0.764(95%,CI为0.633~0.866),其特异性达86.1%;以血清VEGF和AFP为参数,拟合预测PHC发生的Logistic二元回归方程,模型方程拟合优度高(χ2=6.097,P<0.05),预测PHC发生的准确率达78.9%。结论:VEGF和AFP可用于预测PHC发生,如二者联合作为预测因素,其特异性和准确性均较高。
Objective: To investigate the clinical value of vascular endothelial growth factor (VEGF) in the diagnosis of primary hepatocellular carcinoma (PHC). Methods: Twenty-one patients with PHC and 36 patients with cirrhosis were selected as the study objects. Serum VEGF expression was detected by enzyme-linked immunosorbent assay (ELISA), and its correlation with AFP and the value of PHC were analyzed. Results: The serum level of VEGF in patients with PHC was higher than that in patients with liver cirrhosis, and those in patients with cirrhosis were significantly higher than those in healthy controls (Hc = 19.672, P <0.05). Serum VEGF was positively correlated with AFP (r2 = 0.488, P < , AFP predicted receiver operating characteristic curves (ROCs) of 0.819 and 0.774 for PHC, both of which predicted PHC (P <0.05); with VEGF≥106.6 pg / m L and AFP≥30.06 ng / m L as predictors The ROC was 0.764 (95%, CI 0.633 ~ 0.866) with a specificity of 86.1%. The logistic regression equation was fitted to predict the occurrence of PHC by using serum VEGF and AFP as parameters, and the model equation was of goodness of fit (χ2 = 6.097, P <0.05), and the accuracy rate of PHC prediction was 78.9%. Conclusion: VEGF and AFP can be used to predict the occurrence of PHC, as the combination of the two as a predictor, the specificity and accuracy are high.