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目的 :探讨α 生育酚对精索静脉曲张 (VC)大鼠睾丸生殖细胞损害的保护作用。方法 :采用成年雄性Wistar大鼠复制精索静脉曲张模型 ;治疗组 (TG)于手术后 4周开始im ,qdα 生育酚 0 .5mg/(10 0 g体重 )共 4周 ,手术对照组 (CG)动物模型不用药物治疗 ,假手术对照组 (SOG)只行左肾静脉分离 ,不予结扎 ;3组大鼠同等条件饲养 8周。 8周后用原位末端脱氧核苷酸转移酶标记法 (TUNEL)检测睾丸生殖细胞凋亡指数 (AI)。结果 :TG动物左侧睾丸重量显著高于CG(P <0 .0 5 ) ,低于SOG(P <0 .0 5 ) ;TG细胞AI[左侧 (5 .34± 1.2 8) % ,右侧 (5 .87± 1.2 5 ) % ]高于SOG[左侧 (4.72± 1.0 2 ) %、右侧 (4.88± 1.2 8) % ](P <0 .0 5 ) ,而显著低于手术对照组 [左侧 (9.0 5± 2 .5 1) % ,右侧 (7.2 2± 1.35 ) % ](P <0 .0 5 )。结论 :α 生育酚能降低精索静脉曲张大鼠生殖细胞的凋亡指数 ,延缓睾丸细胞损害的病程 ,对精索静脉曲张导致的睾丸损害具有一定的保护作用
Objective: To investigate the protective effects of α-tocopherol on germ cell damage in testis of varicocele (VC) rats. Methods: The varicocele model was established in adult male Wistar rats. The treatment group (TG) started im at 4 weeks after operation, with qdα tocopherol 0.5 mg / (100 g body weight) for 4 weeks. The control group (CG ) Animal model without drug treatment, sham operation control group (SOG) only left renal vein separation, not ligation; 3 rats were fed on the same conditions for 8 weeks. After 8 weeks, testicular germ cell apoptotic index (AI) was detected by TUNEL. Results: The left testes weight of TG animals was significantly higher than CG (P <0.05), lower than that of SOG (P <0.05) (5.87 ± 1.2 5)%] was significantly higher than that of SOG (4.72 ± 1.0 2% on the left and 4.88 ± 1.2 8% on the right) (P <0.05) Group [left (9.0 5 ± 2.51)%, right (7.2 2 ± 1.35)%] (P <0.05). CONCLUSION: Alpha-tocopherol can reduce the apoptosis index of germ cells of rat with varicocele, delay the course of testicular cell damage, and have some protective effect on the testicular damage caused by varicocele