论文部分内容阅读
目的检测肾透明细胞癌(RCCC)组织中肝细胞黏附因子(HepaCAM)的表达水平,探讨HepaCAM在肾透明细胞癌发生、发展过程中的意义。方法选取73例肾透明细胞癌组织和12例癌旁组织作为研究对象,采用非生物素即用型二步法(PV-9001)免疫组织化学方法检测组织中HepaCAM蛋白的表达,运用Image-Pro Plus 6.0图像分析软件测定免疫组化图像的累积光密度值(IOD),应用SPSS13.0软件进行统计分析。结果 HepaCAM蛋白在RCCC组织中低表达,而在癌旁组织中高表达,IOD分别为8203.87±8431.88和36639.28±20851.94,差异具有统计学意义(P<0.05)。而HepaCAM蛋白的表达与性别、年龄、肿瘤大小、T分期和TNM分期等临床特征均无相关关系。结论 HepaCAM蛋白在癌组织中低表达,可能与肾透明细胞癌的发生有密切关系,可作为判断肿瘤发生的指标。
Objective To detect the expression of HepaCAM in renal clear cell carcinoma (RCCC) and to investigate the significance of HepaCAM in the occurrence and development of clear cell renal cell carcinoma (RCCC). Methods Totally 73 cases of renal clear cell carcinoma and 12 cases of paracancerous tissues were selected as the research object. The expression of HepaCAM protein was detected by non-biotin 2-step immunohistochemistry (PV-9001) Plus 6.0 image analysis software for the determination of the cumulative optical density (IOD) of immunohistochemical images, using SPSS13.0 software for statistical analysis. Results The expression of HepaCAM protein was low in RCCC tissues and high in paracancerous tissues with IOD of 8203.87 ± 8431.88 and 36639.28 ± 20851.94, respectively, with statistical significance (P <0.05). The expression of HepaCAM protein had no correlation with clinical features such as sex, age, tumor size, T stage and TNM stage. Conclusion The low expression of HepaCAM protein in cancerous tissues may be closely related to the occurrence of clear cell renal cell carcinoma and may be used as an indicator of tumorigenesis.