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目的:探讨雾化吸入一氧化氮供体亚硝酸钠(Sodium nitrite,NaNO2)后对低氧性肺动脉高压(Hy-poxic ptulmonary hypertension,HPH)大鼠一般状况及血流动力学的影响。方法:雄性Wistar大鼠30只,随机分为3组:常氧空白组,低氧空白组和低氧亚硝酸钠组,每组10只,采用常压低氧法建立大鼠HPH动物模型。测定平均肺动脉压(mPAP)、右室重量、左室+室间隔重量并计算右心室肥大指数(RVHI=RV/LV+S);光镜观察肺小动脉结构改变。结果:低氧前各组大鼠体重无差别,而低氧后大鼠体重差别有统计学意义(P<0.05);低氧组大鼠的mPAP、RVHI值与常氧组相比均升高,低氧亚硝酸钠与低氧空白组比较mPAP和RV/(LV+S)值降低(P<0.05);光镜下,常氧空白组肺动脉管腔结构正常;低氧空白组肺小动脉平滑肌增生,胶原纤维增多,管壁增厚,管腔狭窄;低氧亚硝酸钠组管壁狭窄较低氧空白组有所缓减。结论:雾化吸入亚硝酸钠能有效降低肺动脉压力,减轻右室重构,可能通过直接作用或在体内影响气体信号分子的分泌及合成来实现对HPH的防治。
Objective: To investigate the effects of inhalation of nitric oxide (NO) donor sodium nitrite (NaNO2) on the general condition and hemodynamics of hypoxic pulmonary hypertension (HPH) rats. Methods: Thirty male Wistar rats were randomly divided into 3 groups: normoxia group, hypoxia blank group and hypoxia sodium nitrite group, with 10 rats in each group. The animal model of HPH was established by hypobaric hypoxia. The mean pulmonary arterial pressure (mPAP), right ventricular mass, left ventricular + interventricular septum weight were measured and the right ventricular hypertrophy index (RVHI = RV / LV + S) was calculated. Results: Before hypoxia, body weight of rats in each group had no difference, but body weight of rats in hypoxia had a significant difference (P <0.05); mPAP and RVHI in hypoxia group were higher than those in normoxia group (P <0.05). Compared with hypoxia blank control group, the mPAP and RV / (LV + S) decreased significantly in hypoxia and nitrite groups (P <0.05) Smooth muscle hyperplasia, increased collagen fibers, wall thickening, stenosis; hypoxia sodium nitrite group wall stenosis lower oxygen blank group was reduced. Conclusion: Inhaled sodium nitrite can effectively reduce the pulmonary artery pressure and reduce the remodeling of right ventricle, which may be achieved through direct action or in vivo secretion and synthesis of gas signaling molecules.