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脂肪醇聚氧乙烯醚羧酸和烷基酚聚氧乙烯醚羧酸混合物可根据烷基链的碳链长度用反相离子对高效液相色谱法分离。色谱柱采用SupelcosilTMLC-18,流动相为乙睛:甲醇:水=70:10:20(体积比),含离子对试剂硫酸氢四丁基铵6mmol/L,用氢氧化钠调pH值至6。在选定的色谱条件下,样品各组分之间具有良好的分离效果,该方法有较好的重视性。
The mixture of fatty alcohol polyoxyethylene ether carboxylic acid and alkylphenol polyoxyethylene ether carboxylic acid can be separated by high performance liquid chromatography with reversed-phase ionophoresis according to the carbon chain length of the alkyl chain. The column was equipped with Supelcosil LC-18 and the mobile phase was acetonitrile: methanol: water = 70:10:20 (volume ratio). The ion pair reagent tetrabutylammonium hydrogen sulfate 6mmol / L, the pH was adjusted to 6 with sodium hydroxide . Under the selected chromatographic conditions, the sample has good separation between the components, the method has a good emphasis.