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为寻找一种敏感、特异的丝虫病监测方法，根据班氏丝虫ｐ Ｗ ｂ１２ 重复序列，用聚合酶链反应扩增班氏丝虫特异的 Ｄ Ｎ Ａ 片段（４９０ ｂｐ），回收后用光敏生物素标记该片段，制备成非放射性 Ｄ Ｎ Ａ 探针，并在探针的制备方法上做了新的尝试。实验结果显示，该探针只与班氏微丝蚴及其感染蚊 Ｄ Ｎ Ａ 出现杂交，与马来微丝蚴、正常蚊以及人白细胞的 Ｄ Ｎ Ａ 未出现杂交；检测 ６ 份现场镜检班氏微丝蚴阳性的血样 Ｐ Ｃ Ｒ 产物全部阳性，８ 份正常对照均阴性；与微丝蚴模拟血样本和 Ｄ Ｎ Ａ 样本的 Ｐ Ｃ Ｒ 扩增产物杂交时，敏感性分别达１ 条微丝蚴／１００ μｌ和 ２ ｐｇ；检测人工感染的班氏丝虫阳性蚊虫扩增产物全部阳性，正常蚊全部阴性。
In order to find a sensitive and specific method for the monitoring of filariasis, the Banana filariasis-specific D NA fragment (490 bp) was amplified by polymerase chain reaction (PCR) The fragment was labeled with photo-biotin to prepare a non-radioactive DNA probe, and a new attempt was made to prepare the probe. The results of the experiment showed that the probe only hybridized with B. fasciatus and its infected mosquito D N A and did not hybridize with D. nipponense, normal mosquito and human leukocyte D N A; Bancrofeex positive samples of blood P C R products were all positive, 8 negative controls were negative; and microfilariae simulation blood samples and D N A samples of P C R amplification products hybridization, the sensitivity of up to 1 Microfilariae / 100 μl and 2 pg; all the positive products of positive detection of Bancroftian filariasis in artificial infection were negative, and all the normal mosquitoes were negative.