论文部分内容阅读
目的 建立既符合临床特征、又简便易行的标准大鼠多器官功能障碍综合征(MODS)模型。方法 用人为方法使大鼠失血 0 .5～ 0 .7ml、两后肢粉碎性骨折合并软组织挫裂形成严重复合伤 ,以诱发MODS ;参照临床标准提出大鼠的实验诊断标准 ;观察并检测 84只大鼠和16只对照大鼠在创伤 8、2 4和 48h后生化和病理变化。结果 大鼠创伤 2 4h后 ,肌酐、肌酸磷酸激酶、乳酸脱氢酶、羟丁酸脱氢酶和谷丙转氨酶浓度分别达到 ( 14 0 .3± 34 .4)mol/L、( 14 318.0±2 12 8.9)U /L、( 2 373.7± 2 74.9)U /L、( 1179.5± 2 84.9)U/L和 ( 2 98.2± 40 .6)U /L的最高值 ,与对照组相比差异具有非常显著性 (P <0 .0 1) ;48h后病理变化最明显。创伤后 2 4h的大鼠MODS处于若干脏器功能衰竭早期伴若干脏器功能受损期。结论 本研究的复制方法是成功的 ,复制的MODS模型适于创伤及其药物筛选研究
Objective To establish a model of multiple organ dysfunction syndrome (MODS) that meets clinical characteristics and is simple and easy to operate. Methods Human blood loss of 0.5 ~ 0.7ml was induced by artificial method. The comminuted fractures of both hindlimbs combined with the soft tissue deformity formed a severe combined injury to induce MODS. The experimental diagnostic criteria of rats were proposed according to the clinical criteria. The observation and detection of 84 Rats and 16 control rats had biochemical and pathological changes at 8, 24 and 48 h after trauma. Results After 24 h of trauma, creatinine, creatine phosphokinase, lactate dehydrogenase, hydroxybutyrate dehydrogenase and alanine aminotransferase concentrations were (14.3 ± 34.4) mol / L, (14 318.0 ± 2 12 8.9) U / L, (2 373.7 ± 2 74.9) U / L, (1179.5 ± 2 84.9) U / L and (2 98.2 ± 40.6) U / L, The difference was significant (P <0.01). The pathological changes were the most obvious after 48h. Rats MODS at 24 hours after trauma were in the early stage of several organ failure with some organ dysfunction. Conclusion The replication method in this study was successful. The replicated MODS model was suitable for trauma and drug screening