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【目的】研究儿童噬血细胞性淋巴组织细胞增生症(hemophagocytic lymphohistiocytosis,HLH)外周血淋巴细胞活性的改变。【方法】选择2005年1月—2009年12月在本院住院的49例确诊为HLH且为急性期的患儿为病例组,30例健康儿童为对照组,分别抽取外周血,肝素抗凝,采用流式细胞术方法分别检测两组外周血淋巴细胞CD3+、CD4+、CD8+、CD(16+56)+的表达率。【结果】病例组CD(16+56)+和CD8+细胞比例显著低于对照组(P<0.01),CD3+细胞比例高于对照组(P<0.05),CD4+细胞比例与对照组比较无显著统计学意义(P>0.05)。【结论】NK细胞[CD(16+56)+]和CTL细胞(CD8+)活性的降低对诊断HLH可能具有重要临床意义,T淋巴细胞(CD3+)活性增强对诊断HLH也有一定价值。
【Objective】 To investigate the changes of peripheral blood lymphocyte activity in children with hemophagocytic lymphohistiocytosis (HLH). 【Methods】 Forty-nine children admitted to our hospital from January 2005 to December 2009 were selected as case group and 30 healthy children as control group. Peripheral blood and heparin anticoagulation , Respectively. The expression of CD3 +, CD4 +, CD8 +, CD (16 + 56) + in peripheral blood lymphocytes was detected by flow cytometry. 【Results】 The proportion of CD 16 + 56 cells and CD8 + cells in the case group was significantly lower than that in the control group (P <0.01), and the proportion of CD3 + cells was higher than that in the control group (P <0.05). There was no significant difference in CD4 + Significance (P> 0.05). 【Conclusion】 The decrease of the activity of NK cells [CD (16 + 56)] and CTL cells (CD8 +) may have important clinical significance for the diagnosis of HLH. The enhancement of T lymphocyte (CD3 +) activity is also valuable for the diagnosis of HLH.