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通过正交试验设计优化白芍当归复方的提取工艺,以芍药苷、阿魏酸提取率和浸膏得率为指标,考察乙醇浓度、提取时间、溶媒量和提取次数对工艺的影响,采用HPLC法测定指标性成分芍药苷和阿魏酸的含量。结果显示,最佳提取工艺为:用6倍量的70%乙醇回流提取3次,每次15 min,芍药苷和阿魏酸的提取率为12.99 mg/g(RSD 0.55%)和0.593 mg/g(RSD 2.83%),浸膏得率为42.85%(RSD 1.15%)。
The orthogonal experiment was designed to optimize the extraction process of Radix Angelicae Dangarai. The effects of ethanol concentration, extraction time, amount of solvent and extraction times on the technology were investigated by using the extraction rate of paeoniflorin, ferulic acid and the yield of extract. Determination of the indicators of paeoniflorin and ferulic acid content. The results showed that the optimum extraction conditions were: extraction with 6 times the amount of 70% ethanol reflux three times, each 15 min, the extraction rate of paeoniflorin and ferulic acid 12.99 mg / g (RSD 0.55%) and 0.593 mg / g (RSD 2.83%). The extraction yield was 42.85% (RSD 1.15%).