论文部分内容阅读
目的用一种新的位相变异(诱导)实验方法对沙门菌进行血清型鉴定。方法取1滴(约50μl)诱导血清到直径6 cm的平板中,再加入约5 ml的软琼脂,待凝固后将沙门菌点种到平板中央。37℃培养24 h后,取菌落边缘部分对H因子鉴定。同时,按照GB 4789.4—2010《食品安全国家标准食品微生物学检验沙门氏菌检验》中的简易平板法、倒管法和玻管法进行位相变异实验对H因子进行鉴定。结果 184株沙门菌中有176株按照本方法确定了血清型,有156株经诱导后得到了另一相H因子;而用平板法、倒管法和玻管法分别有77株、120株、112株确定了血清型。本方法的诱导成功率远高于国家标准推荐的平板法、倒管法和小玻管法。结论该方法是一种可靠的沙门菌位相变异实验的方法,可以在日常工作中广泛使用。
Objective To identify serotypes of Salmonella by a new phase mutation (induction) experiment. Methods Take 1 drop (about 50 μl) of the induced serum to a plate of 6 cm in diameter, add about 5 ml of soft agar, and spot the Salmonella into the center of the plate after solidification. After incubation at 37 ℃ for 24 h, the edge of the colony was identified for H factor. At the same time, in accordance with GB 4789.4-2010 “national food safety standards for food microbiology testing Salmonella test” simple flat method, inverted tube method and glass tube method for phase variation experiments to identify H factor. Results Among 184 strains of Salmonella, 176 strains were identified according to this method. 156 strains were obtained after the induction of another phase H factor; and by plate method, inverted tube method and glass tube method, 77 strains and 120 strains , 112 confirmed the serotypes. The success rate of induction of this method is much higher than the national standard recommended plate method, inverted tube method and the small glass tube method. Conclusion This method is a reliable method for the phase-change experiment of Salmonella and can be widely used in daily work.